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cfx maestro software  (Bio-Rad)


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    Structured Review

    Bio-Rad cfx maestro software
    Cfx Maestro Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 4019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maestro+software/CFX+Maestro+Software/pmc13092596-29-0-4
    Average 98 stars, based on 4019 article reviews
    cfx maestro software - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    other:

    Article Title: Development of an RT-qPCR and a Next-Generation Sequencing approach to assess viral shedding of NDV-based SARS-CoV-2 variant vaccines.
    Article Snippet: Slope, efficiency, and the coefficient of determination (R2) were calculated using Maestro Software (Bio-Rad).

    Article Title: <scp>DNA</scp>‐based detection and quantification of <i>Ascochyta rabiei</i> in chickpea (<i>Cicer arietinum</i>) using droplet digital <scp>PCR</scp>
    Article Snippet: The qPCR products were quantified by the simple absolute quantification method described by Brankatschk et al. (2012), using the standard curve and the Maestro software (Bio- Rad).

    Article Title: Distinct cellular and molecular responses to infection in three target cell types from horses, a species naturally susceptible to Ross River virus.
    Article Snippet: A two-step qRT-PCR 843 was performed using QuantiNova PCR Kit (Qiagen) on the CFX96 Touch or CFX Opus CFX96 Real-844 Time PCR machine with the Maestro software (Bio-Rad).

    Article Title: Development of an RT-qPCR and a Next-Generation Sequencing approach to assess viral shedding of NDV-based SARS-CoV-2 variant vaccines
    Article Snippet: Slope, efficiency, and the coefficient of determination ( R 2 ) were calculated using Maestro Software (Bio-Rad).

    Article Title: Microglia modulate concussion biomarkers and cognitive recovery in male mice
    Article Snippet: We used the miRCURY LNA SYBR Green System (Qiagen) to prepare samples according to manufacturer instructions. qPCR was performed on the CFX384 Real-Time PCR System (Bio-Rad) and processed using the accompanying Maestro software (Bio-Rad).

    Software:

    Article Title: Molecular response of Chironomus riparius to antibiotics
    Article Snippet: .. The Maestro software (Bio-Rad, USA) used the regression option to determine the threshold cycle (Ct). ..

    Article Title: Molecular response of Chironomus riparius to antibiotics.
    Article Snippet: .. The Maestro software (Bio-Rad, USA) used the regression option to determine the threshold cycle (Ct). ..

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..

    Negative Control:

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..

    Control:

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..

    Amplification:

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..

    Generated:

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..

    Positive Control:

    Article Title: Phenotypic and molecular characterization of the response to two soil-borne viruses in durum wheat landraces for the identification of new sources of resistance
    Article Snippet: The reaction was performed using the CFX96 Realtime PCR Detection System thermal cycler (Bio-Rad, Hercules, CA, USA) and controlled by the CFX Maestro software. .. In each plate, a negative control (NC) and a no template control (WC) were added, and amplification efficiency (E) was determined using Bio-Rad’s Maestro software, according to the standard curve generated from a dilution series (six dilution points) of a positive control. ..



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    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad <t>CFX</t> <t>Connect</t> <t>Real-Time</t> <t>PCR</t> Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .
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    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad <t>CFX</t> <t>Connect</t> <t>Real-Time</t> <t>PCR</t> Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .
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    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad <t>CFX</t> <t>Connect</t> <t>Real-Time</t> <t>PCR</t> Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .
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    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad <t>CFX</t> <t>Connect</t> <t>Real-Time</t> <t>PCR</t> Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .
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    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .

    Journal: Current Research in Structural Biology

    Article Title: Screening for novel chemical scaffolds targeting PCNA identifies the Hsp90alpha inhibitor SNX-2112

    doi: 10.1016/j.crstbi.2026.100183

    Figure Lengend Snippet: PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .

    Article Snippet: Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM.

    Techniques: Thermal Shift Assay, Control, Real-time Polymerase Chain Reaction, Concentration Assay, Recombinant, Purification, Fluorescence, Negative Control, Produced, Maestro Software

    PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .

    Journal: Current Research in Structural Biology

    Article Title: Screening for novel chemical scaffolds targeting PCNA identifies the Hsp90alpha inhibitor SNX-2112

    doi: 10.1016/j.crstbi.2026.100183

    Figure Lengend Snippet: PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .

    Article Snippet: Thermal shift assays were performed using a BioRad CFX Connect Real-Time PCR Detection System.

    Techniques: Thermal Shift Assay, Control, Real-time Polymerase Chain Reaction, Concentration Assay, Recombinant, Purification, Fluorescence, Negative Control, Produced, Maestro Software